Рад у часопису

објављена верзија

енглески

Aleksandar Mijatović, Angelina Z. Caković, Aleksandar Lolić, Olivera Klisurić, Marko N. Živanović, Dragana S. Šeklić, Snežana Sretenović, Marija Ilić, Jovana Bogojeski

Structure, interaction with biomolecules, and cytotoxicity of copper (II) complexes chelating some Schiff base ligands

Applied Organometallic Chemistry

Wiley

2023

Cancer remains one of the world's deadliest diseases, claiming many lives each day. Transition metal complexes are leading candidates in developing anticancer agens with cisplatin used in chemotherapy worldwide. Copper, an endogenous metal in the human body, is especially attractive because of its typical redox biological activities and a relatively strong affinity to nucleobases. A series of Cu(II) complexes with ethane-1,2-diamine as the amine part and pentane-2,4-dione and/or 1-phenylbutane-1,3-dione, pentane-2,4-dione and/or 1,1,1- trifluoropentane-2,4-dione, or 1,1,1-trifluoropentane-2,4-dione and/or 1-phenylbutane-1,3-dione as the β-diketone part were synthesized. All complexes have been fully characterized by elementary analysis and various spectroscopy methods, such as UV-Vis, IR and NMR. X-ray crystal structure analysis characterized complex 1 containing ethane-1,2-diamin and pentane2,4-dione ligand. The interactions of the studied Cu(II) complexes (1-6) with calf thymus DNA (ctDNA) were investigated by electronic absorption, spectroscopic fluorescence method and viscosity measurements. In contrast, the interaction with Salmon Sperm DNA was investigated using the electrophoretic mobility shift assay. The results indicate moderate DNA binding affinity of the studied complexes by intercalation. Gel electrophoresis also shows that complexes interact with DNA in a concentration-dependent manner. Interactions between
complexes and bovine serum albumin (BSA) were studied by spectroscopic fluorescence techniques. Complexes 1-6 display a relatively high binding ability for the BSA. Cytotoxicity analyses were performed on human colorectal carcinoma HCT-166 and healthy lung fibroblast MRC-5 cell lines, showing that complex 5 exhibits selectivity between cancer and normal cells, which is crucial for drug development.

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10.1002/aoc.7253

0268-2605

Inorganic Chemistry,General Chemistry

Inorganic Chemistry,General Chemistry

https://onlinelibrary.wiley.com/doi/pdf/10.1002/aoc.7253

M20

М21

Затворени приступ

Creative Commons – Attribution-No Derivative Works 4.0 International

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