Саопштење са скупа штампано у изводу

објављена

енглески

leksandar Mijatović, Biljana Petrović, Ratomir Jelić and Živadin Bugarčić

Equilibrium and kinetic studies of the reactions between [Ru(terpy)(bipy)Cl]Cl complex and biologically important N-donor ligands

EuroAnalysis 16 - Challenges in Modern Analytical Chemistry

http://www.euroanalysis2011.rs/

2011

Studije u oblasti antikancerogenih karakteristika jedinjenja rutenijuma(III/II) izazivaju veliku pažnju već nekoliko godina otkako su neka od njih kao što su KP1019 (indazolijum-tetrahloridobis(H-indazol)rutenat(III)) i NAMI-A (imidazolijum) -trans-tetrahlorido(dimetilsulfoksid)indazolerutenat(III)) dostigao nivo kliničkog ispitivanja. Kompleksi rutenijuma u ​​reakciji sa molekulom DNK formiraju prvenstveno gvanin- i adenin-adukt, kao komplekse platine. Takođe, nekoliko studija ukazuje na veoma jake interakcije sa proteinima. Međutim, kompleksi Ru(III/II) pokazuju generalno manje toksične efekte od lekova platine(II).1
Proučavali smo kinetiku reakcija supstitucije kompleksa [Ru(terpi)(bipi)Cl]Cl sa biološki važnim ligandima: dimetilsulfoksidom, gvanozin-5’-monofosfatom, tioureom i L-histidinom. Sve reakcije su proučavane UV-VIS spektrofotometrijom u 0,1 M NaClO4 sa 10 mM NaCl. Veće koncentracije liganada su korišćene da bi se obezbedili uslovi pseudo prvog reda. Konstante brzine i aktivacioni parametri su izračunavani korišćenjem računarskog programa Origin 6.1. Takođe smo istraživali reakcije hidrolize i kompleksiranja Ru(II) kompleksa sa gvanozin-5’-monofosfatom i L-histidinom. Potenciometrijske titracije su izvedene na 298 K u inertnoj atmosferi korišćenjem staklene elektrode. Za proračune je korišćen računarski program HIPERKUAD.

The studies in the field of anticancer characteristics of ruthenium(III/II) compounds have caused much attention for several years since some of them such as KP1019 (indazolium-tetrachloridobis(H-indazole)ruthenate(III)) i NAMI-A (imidazolium-trans-tetrachlorido(dimethylsulfoxide)indazoleruthenate(III)) reached the level of clinical investigation. Ruthenium complexes in the reaction with DNA molecule form preferentially the guanine- and adenine-adduct, as platinum complexes. Also, several studies indicate very strong interactions with proteins. However, Ru(III/II) complexes show generally less toxic effects than platinum(II) drugs.1
We studied the kinetics of the substitution reactions of [Ru(terpy)(bipy)Cl]Cl complex with biologically important ligands: dimethylsulfoxide, guanosine-5’-monophosphate, thiourea and L-histidine . All reactions were studied by UV-VIS spectrophotometry in 0.1 M NaClO4 with 10mM NaCl. The excess of ligand concentration was used to observed pseudo-first order conditions. The rate constants and activation parameters are calculated using computational program Origin 6.1. Also we investigated the hydrolysis and complexation reactions of Ru(II) complex with guanosine-5’-monophosphate and L-histidine. Potentiometric titrations were carried out at 298 K in inert atmosphere using a glass electrode. For the calculations is used computation program HIPERQUAD.

М30

М34

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